Quantitative determination of trimethylamine in urine by solid-phase microextraction and gas chromatography–mass spectrometry

Graham Mills; V. Walker; H. Mughal

doi:10.1016/S0378-4347(98)00542-8

Quantitative determination of trimethylamine in urine by solid-phase microextraction and gas chromatography–mass spectrometry

Graham Mills, V. Walker, H. Mughal

Research output: Contribution to journal › Article › peer-review

Abstract

Trimethylaminuria (fish odour syndrome) is diagnosed from an increase in urinary excretion of trimethylamine with decreased trimethylamine oxide. We report a new quantitative stable isotope dilution gas chromatography–mass spectrometry procedure for the analysis of these metabolites using solid-phase microextraction (SPME). Both polydimethylsiloxane and mixed Carboxen–polydimethylsiloxane SPME fibres were found to be suitable for the headspace extraction of TMA. This new sampling technique could have wide application for the analysis of volatile and semi-volatile compounds by metabolic screening laboratories.

Original language	English
Pages (from-to)	281-285
Number of pages	5
Journal	Journal of Chromatography B: Biomedical Sciences and Applications
Volume	723
Issue number	1-2
DOIs	https://doi.org/10.1016/S0378-4347(98)00542-8
Publication status	Published - 19 Feb 1999

Access to Document

10.1016/S0378-4347(98)00542-8

Cite this

@article{61f954cdf8454ecc82ca3eda33aad17a,

title = "Quantitative determination of trimethylamine in urine by solid-phase microextraction and gas chromatography–mass spectrometry",

abstract = "Trimethylaminuria (fish odour syndrome) is diagnosed from an increase in urinary excretion of trimethylamine with decreased trimethylamine oxide. We report a new quantitative stable isotope dilution gas chromatography–mass spectrometry procedure for the analysis of these metabolites using solid-phase microextraction (SPME). Both polydimethylsiloxane and mixed Carboxen–polydimethylsiloxane SPME fibres were found to be suitable for the headspace extraction of TMA. This new sampling technique could have wide application for the analysis of volatile and semi-volatile compounds by metabolic screening laboratories.",

author = "Graham Mills and V. Walker and H. Mughal",

year = "1999",

month = feb,

day = "19",

doi = "10.1016/S0378-4347(98)00542-8",

language = "English",

volume = "723",

pages = "281--285",

journal = "Journal of Chromatography B: Biomedical Sciences and Applications",

issn = "0378-4347",

publisher = "Elsevier BV",

number = "1-2",

}

TY - JOUR

T1 - Quantitative determination of trimethylamine in urine by solid-phase microextraction and gas chromatography–mass spectrometry

AU - Mills, Graham

AU - Walker, V.

AU - Mughal, H.

PY - 1999/2/19

Y1 - 1999/2/19

N2 - Trimethylaminuria (fish odour syndrome) is diagnosed from an increase in urinary excretion of trimethylamine with decreased trimethylamine oxide. We report a new quantitative stable isotope dilution gas chromatography–mass spectrometry procedure for the analysis of these metabolites using solid-phase microextraction (SPME). Both polydimethylsiloxane and mixed Carboxen–polydimethylsiloxane SPME fibres were found to be suitable for the headspace extraction of TMA. This new sampling technique could have wide application for the analysis of volatile and semi-volatile compounds by metabolic screening laboratories.

AB - Trimethylaminuria (fish odour syndrome) is diagnosed from an increase in urinary excretion of trimethylamine with decreased trimethylamine oxide. We report a new quantitative stable isotope dilution gas chromatography–mass spectrometry procedure for the analysis of these metabolites using solid-phase microextraction (SPME). Both polydimethylsiloxane and mixed Carboxen–polydimethylsiloxane SPME fibres were found to be suitable for the headspace extraction of TMA. This new sampling technique could have wide application for the analysis of volatile and semi-volatile compounds by metabolic screening laboratories.

U2 - 10.1016/S0378-4347(98)00542-8

DO - 10.1016/S0378-4347(98)00542-8

M3 - Article

SN - 0378-4347

VL - 723

SP - 281

EP - 285

JO - Journal of Chromatography B: Biomedical Sciences and Applications

JF - Journal of Chromatography B: Biomedical Sciences and Applications

IS - 1-2

ER -

Quantitative determination of trimethylamine in urine by solid-phase microextraction and gas chromatography–mass spectrometry

Abstract

Access to Document

Fingerprint

Cite this