Manufacturing a nanometre scale surface topography with varying surface chemistry to assess the combined effect on cell behaviour

Brian G. Cousins; Jurgita Zekonyte; Patrick J. Doherty; Michael J. Garvey; Rachel L. Williams

doi:10.1504/IJNBM.2008.016878

Manufacturing a nanometre scale surface topography with varying surface chemistry to assess the combined effect on cell behaviour

Brian G. Cousins, Jurgita Zekonyte, Patrick J. Doherty, Michael J. Garvey, Rachel L. Williams^*

^*Corresponding author for this work

University of Liverpool

Research output: Contribution to journal › Article › peer-review

Abstract

Surface topography and surface chemistry can influence cell adhesion. This study evaluated how the combined effect of topography and chemistry can reduce cell spreading and proliferation. Silica nanoparticulate coatings ranging from 7, 14 and 21 nm in diameter were manufactured on glass substrates and chemically modified using organofunctional siloxanes bearing methyl (-CH₃), sulphydryl (-SH), amine (-NH₂), carboxyl (-COOH) and hydroxyl (-OH) terminal functional groups. Chemical modification of 7 nm silica induced changes in wettability with the advancing angle increasing for all modifications and the receding angle increasing for -CH₃, -NH₂ and -SH group modifications. X-ray Photoelectron Spectroscopy (XPS) revealed that the surface chemistry was altered for all chemically modified surfaces confirming the modification step. The Field Emission Scanning Electron Microscopy (FESEM) demonstrated that the nanoparticulate coating remained intact after each chemical treatment. Optical microscopy from tissue culture experiments revealed changes in the morphology of the cells that could be attributed to the surface topography of the nanoparticulate coatings irrespective of the surface chemistry.

Original language	English
Pages (from-to)	320-338
Number of pages	19
Journal	International Journal of Nano and Biomaterials
Volume	1
Issue number	3
DOIs	https://doi.org/10.1504/IJNBM.2008.016878
Publication status	Published - 1 Jan 2008

Keywords

cell interactions
cell morphology
cell proliferation
colloidal silica
nanoparticles
organosilanes
surface analysis
surface modification

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10.1504/IJNBM.2008.016878

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title = "Manufacturing a nanometre scale surface topography with varying surface chemistry to assess the combined effect on cell behaviour",

abstract = "Surface topography and surface chemistry can influence cell adhesion. This study evaluated how the combined effect of topography and chemistry can reduce cell spreading and proliferation. Silica nanoparticulate coatings ranging from 7, 14 and 21 nm in diameter were manufactured on glass substrates and chemically modified using organofunctional siloxanes bearing methyl (-CH3), sulphydryl (-SH), amine (-NH2), carboxyl (-COOH) and hydroxyl (-OH) terminal functional groups. Chemical modification of 7 nm silica induced changes in wettability with the advancing angle increasing for all modifications and the receding angle increasing for -CH3, -NH2 and -SH group modifications. X-ray Photoelectron Spectroscopy (XPS) revealed that the surface chemistry was altered for all chemically modified surfaces confirming the modification step. The Field Emission Scanning Electron Microscopy (FESEM) demonstrated that the nanoparticulate coating remained intact after each chemical treatment. Optical microscopy from tissue culture experiments revealed changes in the morphology of the cells that could be attributed to the surface topography of the nanoparticulate coatings irrespective of the surface chemistry.",

keywords = "cell interactions, cell morphology, cell proliferation, colloidal silica, nanoparticles, organosilanes, surface analysis, surface modification",

author = "Cousins, {Brian G.} and Jurgita Zekonyte and Doherty, {Patrick J.} and Garvey, {Michael J.} and Williams, {Rachel L.}",

year = "2008",

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doi = "10.1504/IJNBM.2008.016878",

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AU - Doherty, Patrick J.

AU - Garvey, Michael J.

AU - Williams, Rachel L.

PY - 2008/1/1

Y1 - 2008/1/1

N2 - Surface topography and surface chemistry can influence cell adhesion. This study evaluated how the combined effect of topography and chemistry can reduce cell spreading and proliferation. Silica nanoparticulate coatings ranging from 7, 14 and 21 nm in diameter were manufactured on glass substrates and chemically modified using organofunctional siloxanes bearing methyl (-CH3), sulphydryl (-SH), amine (-NH2), carboxyl (-COOH) and hydroxyl (-OH) terminal functional groups. Chemical modification of 7 nm silica induced changes in wettability with the advancing angle increasing for all modifications and the receding angle increasing for -CH3, -NH2 and -SH group modifications. X-ray Photoelectron Spectroscopy (XPS) revealed that the surface chemistry was altered for all chemically modified surfaces confirming the modification step. The Field Emission Scanning Electron Microscopy (FESEM) demonstrated that the nanoparticulate coating remained intact after each chemical treatment. Optical microscopy from tissue culture experiments revealed changes in the morphology of the cells that could be attributed to the surface topography of the nanoparticulate coatings irrespective of the surface chemistry.

AB - Surface topography and surface chemistry can influence cell adhesion. This study evaluated how the combined effect of topography and chemistry can reduce cell spreading and proliferation. Silica nanoparticulate coatings ranging from 7, 14 and 21 nm in diameter were manufactured on glass substrates and chemically modified using organofunctional siloxanes bearing methyl (-CH3), sulphydryl (-SH), amine (-NH2), carboxyl (-COOH) and hydroxyl (-OH) terminal functional groups. Chemical modification of 7 nm silica induced changes in wettability with the advancing angle increasing for all modifications and the receding angle increasing for -CH3, -NH2 and -SH group modifications. X-ray Photoelectron Spectroscopy (XPS) revealed that the surface chemistry was altered for all chemically modified surfaces confirming the modification step. The Field Emission Scanning Electron Microscopy (FESEM) demonstrated that the nanoparticulate coating remained intact after each chemical treatment. Optical microscopy from tissue culture experiments revealed changes in the morphology of the cells that could be attributed to the surface topography of the nanoparticulate coatings irrespective of the surface chemistry.

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KW - cell morphology

KW - cell proliferation

KW - colloidal silica

KW - nanoparticles

KW - organosilanes

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Manufacturing a nanometre scale surface topography with varying surface chemistry to assess the combined effect on cell behaviour

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Keywords

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